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KMID : 0950619960050010049
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Journanl of Allied Health Sciences
1996 Volume.5 No. 1 p.49 ~ p.59
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Evaluation of Assay for High-Density Lipoprotein Cholesterol by Direct Enzymatic Method
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Lee Seung-Gwan
Lee Chang-Kyu
Choi Myung-Jae
Lee Kook-Sung
Ryu Jung-Rok
Kim Sang-Sup
Nam Hyun-Chul
Kim Seung-Hee
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Abstract
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We evaluated a new direct assay for quantifying high-density lipoprotein cholesterol. The total CVs of the method ranged between 3.2% and 10.5%. The recovery rates of this method ranged between 99% and 102%. Correlation study were conducted across direct enzymatic method with phosphotungstate precipitation based method as the reference assay. The correlation statistic obtained for 20 specimens were : HDL-cholesterol direct enzymatic assay (Y, DAIICHI Co.) = 1.05735 × precipitation assay(X, Eiken Co.) － 1.6340. Statistics obtained for 40 specimens wane : HDL-cholesteTol direct enzymatic assay(Y, DAHCHI Co.) = 1.00565 × precipitation assay(X, DAHCHI Co.) ＋ 0.40130. Studies from linear relationship statistics had shown that there were no evidence for difference between two methods. In conclusion, the method evaluated here is simple and reliable for measuring HDL-cholesterol in serum without the need for prior separation of other fractions.
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KEYWORD
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